In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg)

Abstract : The effects of a soluble fraction of light cycle oil (LCO) on haemocyte parameters, phenoloxidase (PO) activity and mRNA expression of immune-related genes, in the Pacific oyster, Crassostrea gigas, were tested after seven days of exposure and two weeks of recovery period. Five polycyclic aromatic hydrocarbons (PAHs) out of ten detected in tank water had bioaccumulated at the end of the contamination period. The concentration of PAHs in oyster tissues decreased during the recovery period and 14 days after the exposure, 69% of bioaccumulated PAHs were detected in contaminated oysters. The exposure induced severe oyster mortality (21%), external and internal green colouration of the shell and a significant decrease of PO activity. The mRNA expression of several genes was altered. As a conclusion, a modulation of immune-related parameters was demonstrated using three different approaches, namely cellular (flow cytometry), biochemical (spectrophotometry) and genomics (gene transcription) in oysters after contact with soluble fraction of LCO.
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Aquatic Toxicology, Elsevier, 2010, 97 (3), pp.196-203. 〈10.1016/j.aquatox.2009.08.005〉
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http://hal.univ-brest.fr/hal-00638192
Contributeur : Catherine Bertignac <>
Soumis le : vendredi 4 novembre 2011 - 11:23:28
Dernière modification le : jeudi 11 janvier 2018 - 06:23:34

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Anne Bado-Nilles, Tristan Renault, Nicole Faury, Stéphane Le Floch, Claire Quentel, et al.. In vivo effects of LCO soluble fraction on immune-related functions and gene transcription in the Pacific oyster, Crassostrea gigas (Thunberg). Aquatic Toxicology, Elsevier, 2010, 97 (3), pp.196-203. 〈10.1016/j.aquatox.2009.08.005〉. 〈hal-00638192〉

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