Diversity and function in microbial mats from the Lucky Strike hydrothermal vent field

Valentin Crépeau 1 Marie-Anne Cambon Bonavita 1 Françoise Lesongeur 1 Henintsoa Randrianalivelo 1 Pierre-Marie Sarradin 2 Jozée Sarrazin 2 Anne Godfroy 1
1 LM2E - Laboratoire de microbiologie des environnements extrêmophiles
2 DEEP/LEP - IFREMER- Département Etude des Ecosystèmes Profonds
Abstract : Diversity and function in microbial mats from the Lucky Strike hydrothermal vent field (Mid-Atlantic Ridge) were investigated using molecular approaches. DNA and RNA were extracted from mat samples overlaying hydrothermal deposits and Bathymodiolus azoricus mussel assemblages. We constructed and analyzed libraries of 16S rRNA gene sequences and sequences of functional genes involved in autotrophic carbon fixation [forms I and II RuBisCO (cbbL/M), ATP-citrate lyase B (aclB)]; methane oxidation [particulate methane monooxygenase (pmoA)] and sulfur oxidation [adenosine-5'-phosphosulfate reductase (aprA) and soxB]. To gain new insights into the relationships between mats and mussels, we also used new domain-specific 16S rRNA gene primers targeting Bathymodiolus sp. symbionts. All identified archaeal sequences were affiliated with a single group: the marine group 1 Thaumarchaeota. In contrast, analyses of bacterial sequences revealed much higher diversity, although two phyla Proteobacteria and Bacteroidetes were largely dominant. The 16S rRNA gene sequence library revealed that species affiliated to Beggiatoa Gammaproteobacteria were the dominant active population. Analyses of DNA and RNA functional gene libraries revealed a diverse and active chemolithoautotrophic population. Most of these sequences were affiliated with Gammaproteobacteria, including hydrothermal fauna symbionts, Thiotrichales and Methylococcales. PCR and reverse transcription-PCR using 16S rRNA gene primers targeted to Bathymodiolus sp. symbionts revealed sequences affiliated with both methanotrophic and thiotrophic endosymbionts.
Keywords : microbial mats Lucky Strike vent field metabolic diversity Bathymodiolus azoricus symbionts sulfur oxidation methane oxidation
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Article dans une revue
FEMS Microbiology Ecology, Wiley-Blackwell, 2011, 76 (3), pp.524-540. 〈10.1111/j.1574-6941.2011.01070.x〉
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http://hal.univ-brest.fr/hal-00617962
Contributeur : Violaine Garguilo <>
Soumis le : mercredi 31 août 2011 - 10:38:45
Dernière modification le : jeudi 11 janvier 2018 - 06:16:39

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Valentin Crépeau, Marie-Anne Cambon Bonavita, Françoise Lesongeur, Henintsoa Randrianalivelo, Pierre-Marie Sarradin, et al.. Diversity and function in microbial mats from the Lucky Strike hydrothermal vent field. FEMS Microbiology Ecology, Wiley-Blackwell, 2011, 76 (3), pp.524-540. 〈10.1111/j.1574-6941.2011.01070.x〉. 〈hal-00617962〉

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