Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl PHAs) from cosmetic co-products by Pseudomonas raguenesii sp. nov., isolated from Tetiaroa, French Polynesia

Christelle Simon-Colin 1, * Karine Alain 2 G. Raguénès 1 S. Schmitt 1 N. Kervarec 3 C. Gouin 1 P. Crassous 4 B. Costa 5 J. G. Guezennec 1
* Auteur correspondant
1 Centre de Brest, BIOMAR/BMM
2 LM2E - Laboratoire de microbiologie des environnements extrêmophiles
3 Service Commun RMN-RPE
4 Centre de Brest, EEP/LME
5 Pacific Biotech
Abstract : A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus and the name of Pseudomonas raguenesii sp. nov. is proposed.
Keywords : Microbial mat Pseudomonas mcl PHAs Polyhydroxyalkanoates Co-products
Type de document :
Article dans une revue
Bioresource Technology, Elsevier, 2009, 100 (23), pp.6033-6039. 〈10.1016/j.biortech.2009.06.075〉
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http://hal.univ-brest.fr/hal-00561600
Contributeur : Violaine Garguilo <>
Soumis le : mardi 1 février 2011 - 15:10:41
Dernière modification le : jeudi 11 janvier 2018 - 06:16:39

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Christelle Simon-Colin, Karine Alain, G. Raguénès, S. Schmitt, N. Kervarec, et al.. Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl PHAs) from cosmetic co-products by Pseudomonas raguenesii sp. nov., isolated from Tetiaroa, French Polynesia. Bioresource Technology, Elsevier, 2009, 100 (23), pp.6033-6039. 〈10.1016/j.biortech.2009.06.075〉. 〈hal-00561600〉

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