Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry - Université de Bretagne Occidentale Access content directly
Journal Articles Journal of Clinical Microbiology Year : 2009

Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Abstract

Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.

Domains

Bacteriology
Fichier principal
Vignette du fichier
JCM00175-09_2_1_.pdf (87.94 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

hal-00557578 , version 1 (21-01-2011)

Identifiers

Cite

Marie-Frédérique Lartigue, Geneviève Héry-Arnaud, Eve Haguenoer, Anne-Sophie Domelier, Pierre-Olivier Schmit, et al.. Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology, 2009, 47 (7), pp.2284-2287. ⟨10.1128/JCM.00175-09⟩. ⟨hal-00557578⟩
267 View
582 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More