Identification of secreted and surface proteins from Enterococcus faecalis.

Abdellah Benachour 1, * Thierry Morin 1 Laurent Hébert 1 Aurélie Budin-Verneuil 1 André Le Jeune 1 Yanick Auffray 1 Vianney Pichereau 1, 2
* Auteur correspondant
1 LME - Laboratoire de Microbiologie de l’Environnement
2 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
Abstract : Secreted and surface proteins of bacteria are key molecules that interface the cell with the environment. Some of them, corresponding to virulence factors, have already been described for Enterococcus faecalis, the predominant species involved in enterococcal nosocomial infections. In a global proteomic approach, the identification of the most abundant secreted and surface-associated proteins of E. faecalis JH2-2 strain was carried out. These proteins were separated by gel electrophoresis or directly subjected to in vivo trypsinolysis and then analyzed by liquid chromatography - electrospray ion trap tandem mass spectrometry. Putative functions were assigned by homology to the translated genomic database of E. faecalis. A total of 44 proteins were identified, eight secreted proteins from the supernatant culture and 38 cell surface proteins from two-dimensional gel electrophoresis and in vivo trypsinolysis among which two are common to the two groups. Their sequences analysis revealed that 35 of the 44 proteins harbour characteristic features (signal peptide or transmembrane domains) consistent with an extracellular localization. This study may be considered as an important step to encourage proteomic-based investigations of E. faecalis cell surface associated proteins that could lead to the discovery of virulence factors and to the development of new therapeutic tools.
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Article dans une revue
Canadian Journal of Microbiology, NRC Research Press, 2009, 55 (8), pp.967-974. 〈10.1139/w09-052〉
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http://hal.univ-brest.fr/hal-00455470
Contributeur : Vianney Pichereau <>
Soumis le : mercredi 10 février 2010 - 14:51:03
Dernière modification le : mercredi 6 juin 2018 - 01:23:51

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Abdellah Benachour, Thierry Morin, Laurent Hébert, Aurélie Budin-Verneuil, André Le Jeune, et al.. Identification of secreted and surface proteins from Enterococcus faecalis.. Canadian Journal of Microbiology, NRC Research Press, 2009, 55 (8), pp.967-974. 〈10.1139/w09-052〉. 〈hal-00455470〉

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