A novel DFNB1 deletion allele supports the existence of a distant cis-regulatory region that controls GJB2 and GJB6 expression.

Abstract : Eleven affected members of a large German-American family segregating recessively inherited, congenital, non-syndromic sensorineural hearing loss (SNHL) were found to be homozygous for the common 35delG mutation of GJB2, the gene encoding the gap junction protein Connexin 26. Surprisingly, four additional family members with bilateral profound SNHL carried only a single 35delG mutation. Previously, we demonstrated reduced expression of both GJB2 and GJB6 mRNA from the allele carried in trans with that bearing the 35delG mutation in these four persons. Using array comparative genome hybridization (array CGH), we have now identified on this allele a deletion of 131.4 kb whose proximal breakpoint lies more than 100 kb upstream of the transcriptional start sites of GJB2 and GJB6. This deletion, del(chr13:19,837,344-19,968,698), segregates as a completely penetrant DFNB1 allele in this family. It is not present in 528 persons with SNHL and monoallelic mutation of GJB2 or GJB6, and we have not identified any other candidate pathogenic copy number variation by arrayCGH in a subset of 10 such persons. Characterization of distant GJB2/GJB6 cis-regulatory regions evidenced by this allele may be required to find the 'missing' DFNB1 mutations that are believed to exist.
Type de document :
Article dans une revue
Clinical Genetics, Wiley, 2010, 78 (3), pp.267-74
Liste complète des métadonnées

http://hal.univ-brest.fr/hal-01315457
Contributeur : Catherine Bertignac <>
Soumis le : vendredi 13 mai 2016 - 11:32:53
Dernière modification le : vendredi 12 janvier 2018 - 01:58:28

Identifiants

Citation

E Wilch, H Azaiez, R A Fisher, J Elfenbein, A Murgia, et al.. A novel DFNB1 deletion allele supports the existence of a distant cis-regulatory region that controls GJB2 and GJB6 expression.. Clinical Genetics, Wiley, 2010, 78 (3), pp.267-74. 〈hal-01315457〉

Partager

Métriques

Consultations de la notice

57