Impact of 20 : 4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas

Abstract : Arachidonic acid (20:4n-6, ArA) and its eicosanoid metabolites have been demonstrated to be implicated in immune functions of vertebrates, fish, and insects. Thus, the aim of this study was to assess the impact of ArA supplementation on the FA composition and hemocyte parameters of oysters Crassostrea gigas. Oyster dietary conditioning consisted of direct addition of ArA solutions at a dose of 0, 0.25, or 0.41 mu g ArA per mL of seawater into tanks in the presence or absence of T-Iso algae. Results showed significant incorporation of ArA into gill polar lipids when administered with algae (up to 19.7%) or without algae (up to 12.1%). ArA supplementation led to an increase in hemocyte numbers, phagocytosis, and production of reactive oxygen species by hemocytes from ArA-supplemented oysters. Moreover, the inhibitory effect of Vibrio aestuarianus extracellular products on the adhesive proprieties of hemocytes was lessened in oysters fed ArA-supplemented T-Iso. All changes in oyster hemocyte parameters reported in the present study suggest that ArA and/or eicosanoid metabolites affect oyster hemocyte functions.
Type de document :
Article dans une revue
Lipids, Springer Verlag, 2006, 41 (6), pp.567-576. 〈10.1007/s11745-006-5006-9〉
Liste complète des métadonnées

http://hal.univ-brest.fr/hal-00670289
Contributeur : Catherine Bertignac <>
Soumis le : mercredi 15 février 2012 - 10:27:10
Dernière modification le : jeudi 11 janvier 2018 - 06:22:24

Identifiants

Citation

Maryse Delaporte, Philippe Soudant, Jeanne Moal, Emilie Giudicelli, Christophe Lambert, et al.. Impact of 20 : 4n-6 supplementation on the fatty acid composition and hemocyte parameters of the Pacific oyster Crassostrea gigas. Lipids, Springer Verlag, 2006, 41 (6), pp.567-576. 〈10.1007/s11745-006-5006-9〉. 〈hal-00670289〉

Partager

Métriques

Consultations de la notice

192