Rhizosphere persistence of three Pythium oligandrum strains in tomato soilless culture assessed by DNA macroarrayand real-time PCR

Abstract : In tomato soilless culture, plant-disease optimal control and growth promotion are achieved when the rhizosphere is heavily colonized by the biocontrol agent Pythium oligandrum. Discrepancies in performance are generally attributed to the poor persistence of P. oligandrum on roots. In this study, three selected strains of P. oligandrum were introduced into the rhizosphere of greenhouse-grown tomato plants, and their persistence was assessed by DNA macroarray hybridization and real-time PCR. The experimental data from DNA detection and plate counting were compared. PCR-based methods detected P. oligandrum throughout the 6-month growing season, whereas plate counting indicated its presence only over the first 3 months. Moreover, the DNA array method provided information about the various Pythium species present in the rhizosphere: P. dissotocum was frequently detected on roots of plants, without distinction between plants inoculated or not inoculated with the antagonist. The detection of other Pythium species was noticed sporadically (P. ultimum, P. sylvaticum and P. intermedium), independent of the treatment. Even though the yield enhancement is not significant throughout the entire growing season, data obtained from epidemiological studies demonstrate an enhancement of P. oligandrum persistence on the rhizosphere of plants and less use of mycoparasitism.
Type de document :
Article dans une revue
FEMS Microbiology Ecology, Wiley-Blackwell, 2007, 61 (2), pp.317-326. 〈10.1111/j.1574-6941.2007.00348.x〉
Liste complète des métadonnées

http://hal.univ-brest.fr/hal-00554952
Contributeur : Violaine Garguilo <>
Soumis le : mardi 11 janvier 2011 - 18:12:29
Dernière modification le : mercredi 10 janvier 2018 - 14:42:39

Identifiants

Collections

Citation

Gaétan Le Floch, James Tambong, Jessica Vallance, Yves Tirilly, André Lévesque, et al.. Rhizosphere persistence of three Pythium oligandrum strains in tomato soilless culture assessed by DNA macroarrayand real-time PCR. FEMS Microbiology Ecology, Wiley-Blackwell, 2007, 61 (2), pp.317-326. 〈10.1111/j.1574-6941.2007.00348.x〉. 〈hal-00554952〉

Partager

Métriques

Consultations de la notice

61